Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Differential expression of pyroptosis-related miRNAs in HCC and normal tissues. ( A ) Volcano plot showing differentially expressed miRNAs (DEmiRNAs) in normal (N) and tumor (T) tissues. Red dots represent up-regulated miRNAs, and green dots represent down-regulated miRNAs. ( B ) Heatmap displaying the top 20 differentially expressed miRNAs between HCC and normal tissues

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Construction of risk scores for prognostic models based on pyroptosis-related miRNAs. ( A ) Univariate Cox regression analysis used to identify 10 prognosis-related miRNAs. ( B ) Forest plot summarizing the results of multivariate Cox regression analysis. ( C-D ) Least absolute shrinkage and selection operator (LASSO) regression analysis for selecting the optimal miRNAs associated with overall survival

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques: Selection

Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Validation of the pyroptosis-related miRNA prognostic model. ( A ) PCA plot of HCC patients grouped by risk scores. ( B ) Distribution of risk scores for the patient cohort. ( C ) Survival status distribution of patients. ( D ) Kaplan-Meier survival curves comparing low-risk and high-risk groups. ( E ) ROC curves for predicting 1-, 3-, and 5-year overall survival. ROC analysis was used to calculate AUC values. ( F ) Calibration curves for 1-, 3-, and 5-year overall survival. (Number of High-Risk Samples: 183. Number of Low-Risk Samples: 183)

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques:

Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 34. SARS-CoV-2 RNA contamination on surfaces of a COVID-19 ward in a hospital of Northern Italy: what risk of transmission? , Italy , No , Surface samples from ward in University Hospital of Ferrara , Sampling performed with sterile rayon swabs pre-moistened in sterile phosphate-buffered solution , Viral RNA extraction with Patho Gene-spin Extraction kit (Generon) RT-qPCR targeted the RNA-dependent RNA polymerase ( RdRp ) gene (Generon), and the orf1ab , spike ( S ), and nucleocapsid ( N ) genes (ThermoFisher) , • SARS-CoV-2 was only detected in 3 samples of two floors and one-bathroom sink. • Reported to persist for a longer duration on surfaces under controlled laboratory conditions. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

Journal: Nature cell biology

Article Title: Stomach-derived human insulin-secreting organoids restore glucose homeostasis

doi: 10.1038/s41556-023-01130-y

Figure Lengend Snippet: a, Diagram showing key steps of GINS organoid generation. Medium supplements for hGSC (human gastric stem cell) culture or GINS organoid induction are indicated, with up and down arrows indicating agonists or antagonists, respectively. b, Representative images of human stomach samples, hGSC colonies, and GINS organoids. 1000 cells per organoids. c, d, Immunofluorescent staining and quantification of day-21 GINS organoids for C-peptide (CPPT), MAFA, glucagon (GCG), somatostatin (SST), and ghrelin (GHRL). For CPPT+ cell quantification, n = 33 independent organoids (donor #6), for other hormone quantification, n = 6 independent organoids (donor #6). e, Quantitative flow cytometry of day-21 GINS organoids (donor #10) with negative control. f, Insulin content of day-18 GINS organoids (n = 11 different batches of organoids from donor #6) and human islets (n = 3 samples from independent donors). Data are mean ± s.d.; two-tailed unpaired t-test.

Article Snippet: Fixed cells were then incubated in 1X permeabilization buffer with primary antibodies for 1 h at room temperature and washed with permeabilization buffer for 3 times and resuspended in flow cytometry staining buffer (Thermo Scientific, 00–4222).

Techniques: Stem Cell Culture, Staining, Flow Cytometry, Negative Control, Two Tailed Test